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Serious Adverse Events: Are SAEs as underreported as …
Serious Adverse Events: Are SAEs as underreported as …

In this study, we identified the regulon of a two-component signal transduction system, SaeRS, and demonstrated that the SaeRS system is required for S. aureus to cause infection both in vitro and ...

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Dietary Supplements_百度文库
Dietary Supplements_百度文库

2019-12-31 · Bacterial Cultures, RNA Isolation, and Expression Analysis S. aureus strains were grown in Luria-Bertani medium and then harvested. Cells were isolated by centrifugation and dissolved in a solution of 33 mmol/L sodium acetate, 17 mmol/L sodium dodecyl sulfate, and 1 mmol/L EDTA (pH 5.5).

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Single-dose intravenous iron for iron deficiency: a new ...
Single-dose intravenous iron for iron deficiency: a new ...

High-molecular-weight genomic DNA from bacterial cells was prepared using guanidinium isothiocyanate-isobutanol precipitation on silica to purify DNA from a precleared proteinase K-sodium dodecyl sulfate incubation. Genomic DNA was sheared, concentrated, and desalted using standard protocols.

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The Staphylococcus aureus superantigen SElX is a ...
The Staphylococcus aureus superantigen SElX is a ...

In the Gram-positive pathogenic bacterium Staphylococcus aureus, the SaeRS two-component system (TCS) plays a major role in controlling the production of over 20 virulence factors including hemolysins, leukocidins, superantigens, surface proteins, and proteases.The SaeRS TCS is composed of the sensor histidine kinase SaeS, response regulator SaeR, and two auxiliary proteins SaeP and SaeQ.

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Turquoise Energy
Turquoise Energy

2013-11-5 · We were able to replicate an inflammation-based murine model of tumorigenesis in specific-pathogen-free (SPF) C57BL/6 mice (n = 12) using an intraperitoneal injection of the chemical carcinogen azoxymethane (AOM) followed by three subsequent rounds of water-administered 2% dextran sodium sulfate (DSS) treatment (26, 27) (Fig. 1A).

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Avalon Naturals Itch & Flake Therapy Shampoo, 14 oz …
Avalon Naturals Itch & Flake Therapy Shampoo, 14 oz …

sodium citrate, pH 7), and fixed by baking the membranes at 80°C for 2 h. Membranes were prehybridized in 5 SSC, 0.5% sodium dodecyl sulfate, and 5 Denhardt’s solution (0.1% bovine serum albumin, 0.1% Ficoll 400, 0.1% poly-vinylpyrrolidone) for4hat65°C. Purified DNA …

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The Lead-Acid Battery Longevity Additive
The Lead-Acid Battery Longevity Additive

The SaeRS two-component regulatory system of Staphylococcus aureus is known to affect the expression of many genes. The SaeS protein is the histidine kinase responsible for phosphorylation of the response regulator SaeR. In S. aureus Newman, the sae system is constitutively expressed due to a point mutation in saeS, relative to other S. aureus strains, which results in substitution of proline ...

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Host-derived fatty acids activate type VII secretion in ...
Host-derived fatty acids activate type VII secretion in ...

biocide Perform and the detergent sodium dodecyl sulfate (SDS) activate sae signaling only in strain Newman, suggesting * Corresponding author. Mailing address: Department of Microbi-ology and Immunology, Indiana University School of Medicine-North- ... 18. saeRS. 2011.

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Solution Structures of Phenol-Soluble Modulins α1, …
Solution Structures of Phenol-Soluble Modulins α1, …

Read "Transient diabetes insipidus in a dog with acromegaly, Journal of Small Animal Practice" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

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(PDF) SDS Interferes with SaeS Signaling of …
(PDF) SDS Interferes with SaeS Signaling of …

Sears has the best selection of Conditioner in stock. Get the Conditioner you want from the brands you love today at Sears.

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Fluorescently labeled cDNA was purified by the Qiagen PCR purification kit. After being dried, the cDNA was dissolved in a hybridization buffer composed of 30% formamide, 5× SSC (1× SSC is 0.15 M NaCl plus 0.015 M sodium citrate), 0.1% sodium dodecyl sulfate (SDS), and a 1:100 volume of sheared salmon sperm DNA and heated at 98°C for 2 min.

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Role of the SaeRS two-component regulatory system …
Role of the SaeRS two-component regulatory system …

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that a protein band of approximately 30 kDa was upregulated in subinhibitory concentrations of OXA-, MET-, FOX-, IMI-, or MER-treated N315 compared with the untreated control . In contrast, CHL, VAN, KAN, and ERY showed no induction effects on this protein band.

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In the Staphylococcus aureus Two-Component …
In the Staphylococcus aureus Two-Component …

Chitosan (CH) hydrogels with remarkable mechanical properties and rapid gelation rate were recently synthesized by combining sodium hydrogen carbonate (SHC) with another weak base, such as beta-glycerophosphate (BGP). To improve their biological responses, in the present study, chondroitin sulfate (CS) was added to these CH hydrogels.

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Biological activity and environmental impact of ...
Biological activity and environmental impact of ...

Resistance of Staphylococcus aureus to beta-lactam antibiotics has led to increasing use of the glycopeptide antibiotic vancomycin as a life-saving treatment for major S. aureus infections. Coinfection by an unrelated bacterial species may necessitate concurrent treatment with a second antibiotic that targets the coinfecting pathogen. While investigating factors that affect bacterial ...

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SaeR Binds a Consensus Sequence within Virulence …
SaeR Binds a Consensus Sequence within Virulence …

2016-10-3 · In the Gram-positive pathogenic bacterium Staphylococcus aureus, the SaeRS two-component system (TCS) plays a major role in controlling the production of over 20 virulence factors including hemolysins, leukocidins, superantigens, surface proteins, and proteases.The SaeRS TCS is composed of the sensor histidine kinase SaeS, response regulator SaeR, and two auxiliary proteins …

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